Rapid PCR Detection of Staphylococcus aureus Clonal Complex 398 by Targeting the Restriction-Modification System Carrying sau1-hsdS1

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.

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Bibliographic Details
Main Authors: Stegger, M., Lindsay, J.A., Moodley, A., Skov, R., Broens, E.M., Guardabassi, L.
Format: Article/Letter to editor biblioteca
Language:English
Subjects:eburst, lineages,
Online Access:https://research.wur.nl/en/publications/rapid-pcr-detection-of-staphylococcus-aureus-clonal-complex-398-b
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Description
Summary:A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.