Vitamin A equivalency of ß-carotene in healthy adults: limitation of the extrinsic dual-isotope dilution technique to measure matrix effect
Data on the vitamin A equivalency of ß-carotene in food are inconsistent. We quantified the vitamin A equivalency (¿g) of ß-carotene in two diets using the dual-isotope dilution technique and the oral¿faecal balance technique. A diet-controlled, cross-over intervention study was conducted in twenty-four healthy adults. Each subject followed two diets for 3 weeks each: a diet containing vegetables low in ß-carotene with supplemental ß-carotene in salad dressing oil ('oil diet') and a diet containing vegetables and fruits high in ß-carotene ('mixed diet'). During all 6 weeks, each subject daily consumed a mean of 55 (sd 0·5) ¿g [13C10]ß-carotene and 55 (sd 0·5) ¿g [13C10]retinyl palmitate in oil capsules. The vitamin A equivalency of ß-carotene was calculated as the dose-corrected ratio of [13C5]retinol to [13C10]retinol in serum and from apparent absorption by oral-faecal balance. Isotopic data quantified a vitamin A equivalency of [13C10]ß-carotene in oil of 3·4 ¿g (95 % CI 2·8, 3·9), thus the bio-efficacy of the ß-carotene in oil was 28 % in the presence of both diets. However, data from oral-faecal balance estimated vitamin A equivalency as 6:1 ¿g (95 % CI 4, 7) for ß-carotene in the 'oil diet'. ß-Carotene in the 'oil diet' had 2·9-fold higher vitamin A equivalency than ß-carotene in the 'mixed diet'. In conclusion, this extrinsic labelling technique cannot measure effects of mixed vegetables and fruits matrices, but can measure precisely the vitamin A equivalency of the ß-carotene in oil capsules
| Main Authors: | , , , , , , , , , , |
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | bioavailability, bioconversion, consumption, dark-green, humans, leafy vegetables, mass-spectrometry, retinol, serum, spinach, |
| Online Access: | https://research.wur.nl/en/publications/vitamin-a-equivalency-of-ß-carotene-in-healthy-adults-limitation- |
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| Summary: | Data on the vitamin A equivalency of ß-carotene in food are inconsistent. We quantified the vitamin A equivalency (¿g) of ß-carotene in two diets using the dual-isotope dilution technique and the oral¿faecal balance technique. A diet-controlled, cross-over intervention study was conducted in twenty-four healthy adults. Each subject followed two diets for 3 weeks each: a diet containing vegetables low in ß-carotene with supplemental ß-carotene in salad dressing oil ('oil diet') and a diet containing vegetables and fruits high in ß-carotene ('mixed diet'). During all 6 weeks, each subject daily consumed a mean of 55 (sd 0·5) ¿g [13C10]ß-carotene and 55 (sd 0·5) ¿g [13C10]retinyl palmitate in oil capsules. The vitamin A equivalency of ß-carotene was calculated as the dose-corrected ratio of [13C5]retinol to [13C10]retinol in serum and from apparent absorption by oral-faecal balance. Isotopic data quantified a vitamin A equivalency of [13C10]ß-carotene in oil of 3·4 ¿g (95 % CI 2·8, 3·9), thus the bio-efficacy of the ß-carotene in oil was 28 % in the presence of both diets. However, data from oral-faecal balance estimated vitamin A equivalency as 6:1 ¿g (95 % CI 4, 7) for ß-carotene in the 'oil diet'. ß-Carotene in the 'oil diet' had 2·9-fold higher vitamin A equivalency than ß-carotene in the 'mixed diet'. In conclusion, this extrinsic labelling technique cannot measure effects of mixed vegetables and fruits matrices, but can measure precisely the vitamin A equivalency of the ß-carotene in oil capsules |
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