Methods of detecting root knot nematodes

The present invention relates to a method method of testing a sample for the presence of a Meloidogyne nematode, said method comprising the step of analyzing the nucleic acid in said sample for the presence of an rRNA gene of a Meloidogyne nematode, or the transcription product thereof, or a fragment thereof, comprising at least one single nucleotide polymorphism (SNP) and/or at least one oligonucleotide polymorphism (OP) that is essentially unique on phylum level to a specific group of Meloidogyne nematode species of which said Meloidogyne nematode is a member, wherein said step of analyzing said nucleic acid involves a nucleic acid hybridization assay, and wherein said group comprises (a) the species M. chitwoodi, M. fallax </i

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Bibliographic Details
Main Authors: Helder, J., Karssen, G., van den Elsen, S.J.J., Holterman, M.H.M., Veenhuizen, P.T.M., Landeweert, R., Hekman, H., Bakker, J.
Format: Patent biblioteca
Language:English
Subjects:Life Science,
Online Access:https://research.wur.nl/en/publications/methods-of-detecting-root-knot-nematodes
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Description
Summary:The present invention relates to a method method of testing a sample for the presence of a Meloidogyne nematode, said method comprising the step of analyzing the nucleic acid in said sample for the presence of an rRNA gene of a Meloidogyne nematode, or the transcription product thereof, or a fragment thereof, comprising at least one single nucleotide polymorphism (SNP) and/or at least one oligonucleotide polymorphism (OP) that is essentially unique on phylum level to a specific group of Meloidogyne nematode species of which said Meloidogyne nematode is a member, wherein said step of analyzing said nucleic acid involves a nucleic acid hybridization assay, and wherein said group comprises (a) the species M. chitwoodi, M. fallax </i