Alternatives for forage evaluation in ruminants
The objective of this thesis was to validate and to compare in situ and in vitro techniques with in vivo data. These techniques were also evaluated for future and practical use in feed evaluation for ruminants. The techniques were compared using the digestion data of 98 forages and the energy values: in vivo total tract organic matter digestibility (OMD) and rumen fermentable organic matter (FOM), and the protein values: microbial nitrogen synthesised in the rumen (MNS) and rumen escape N (REN). The in situ nylon bag technique, the pepsin-cellulase technique, the gas production technique (GPT) and the Tilley and Terry technique showed good potency to predict OMD and the in situ technique and GPT gave most accurate FOM predictions. MNS was evaluated using in vivo data from the literature and showed a significant relationship with the content of crude protein in forages. The method of conservation of forages had an extra effect on MNS. REN was determined using in situ and different in vitro techniques, but only ADIN (fibre attached N) had a good relationship with in vivo
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| Format: | Doctoral thesis biblioteca |
| Language: | English |
| Subjects: | alternative methods, animal feeding, animal nutrition, digestibility, experiments, feed evaluation, forage, in vitro, in vivo experimentation, nutrition physiology, rumen digestion, ruminants, techniques, alternatieve methoden, diervoedering, diervoeding, experimenten, herkauwers, in vivo experimenten, pensvertering, ruwvoer (forage), technieken, verteerbaarheid, voederwaardering, voedingsfysiologie, |
| Online Access: | https://research.wur.nl/en/publications/alternatives-for-forage-evaluation-in-ruminants |
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| Summary: | The objective of this thesis was to validate and to compare in situ and in vitro techniques with in vivo data. These techniques were also evaluated for future and practical use in feed evaluation for ruminants. The techniques were compared using the digestion data of 98 forages and the energy values: in vivo total tract organic matter digestibility (OMD) and rumen fermentable organic matter (FOM), and the protein values: microbial nitrogen synthesised in the rumen (MNS) and rumen escape N (REN). The in situ nylon bag technique, the pepsin-cellulase technique, the gas production technique (GPT) and the Tilley and Terry technique showed good potency to predict OMD and the in situ technique and GPT gave most accurate FOM predictions. MNS was evaluated using in vivo data from the literature and showed a significant relationship with the content of crude protein in forages. The method of conservation of forages had an extra effect on MNS. REN was determined using in situ and different in vitro techniques, but only ADIN (fibre attached N) had a good relationship with in vivo |
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