The prohibitin genes in Arabidopsis thaliana: Expression in seeds, hormonal regulation and possible role in cell cycle control during seed germination
A fragment encoding a partial sequence of a prohibitin (Phb) gene was isolated. The expression of Phb mRNA and protein in seeds of wild type and mutant Arabidopsis thaliana seeds is presented. Phb mRNA is abundant in wild-type seeds; thus, it may have sequence or structural characteristics responsible for this stability. The 30 untranslated region sequence of a Phb gene has interesting features. We found that Arabidopsis Phb does not interact with a retinoblastoma-related protein or E2F in a yeast two-hybrid system, thus suggesting that the plant protein may have not conserved such interaction, described for mammalian Phb. The possible role of Phb in cell cycle regulation during germination is discussed.
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| Main Authors: | , , , |
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| Format: | artículo biblioteca |
| Published: |
Elsevier
2007
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| Subjects: | Arabidopsis, prohibitin, post-transcrioptional regulation, seed, 3'-UTR, |
| Online Access: | http://hdl.handle.net/10261/5943 |
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| Summary: | A fragment encoding a partial sequence of a prohibitin (Phb) gene was isolated. The
expression of Phb mRNA and protein in seeds of wild type and mutant Arabidopsis
thaliana seeds is presented. Phb mRNA is abundant in wild-type seeds; thus, it may
have sequence or structural characteristics responsible for this stability. The 30
untranslated region sequence of a Phb gene has interesting features. We found that
Arabidopsis Phb does not interact with a retinoblastoma-related protein or E2F in a
yeast two-hybrid system, thus suggesting that the plant protein may have not
conserved such interaction, described for mammalian Phb. The possible role of Phb
in cell cycle regulation during germination is discussed. |
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