Nocardioides albertani sp. nov., isolated from Roman catacombs
A Gram-positive, aerobic, non-spore-forming, rod- or coccoid-shaped, strain CD40127T, was isolated from a green biofilm covering the wall of the Domitilla Catacombs in Rome, Italy. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CD40127T belongs to the genus Nocardioides, closely related to Nocardioides luteus DSM 43366T and Nocardioides albus DSM 43109T with 98.86% and 98.01% similarity values, respectively. Strain CD40127T exhibited 16S rRNA gene sequence similarity values below 96.29% with the rest of Nocardioides species. The G+C content of the genomic DNA was 69.7 mol%. The predominant fatty acid was iso-C16:0 and the major menaquinone was MK-8 (H4) according to the genus Nocardioides. A polyphasic approach using physiological tests, fatty acid profiles, DNA base ratios and DNA-DNA hybridization showed that isolate CD40127T belongs to a novel species within the genus Nocardioides, for which the name Nocardioides albertani is proposed. The type strain is CD40127T (=DSM 25218T =CECT 8014T).
| Main Authors: | , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Society for General Microbiology
2012
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| Online Access: | http://hdl.handle.net/10261/64982 |
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| Summary: | A Gram-positive, aerobic, non-spore-forming, rod- or coccoid-shaped, strain CD40127T, was isolated from a green biofilm covering the wall of the Domitilla Catacombs in Rome, Italy. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CD40127T belongs to the genus Nocardioides, closely related to Nocardioides luteus DSM 43366T and Nocardioides albus DSM 43109T with 98.86% and 98.01% similarity values, respectively. Strain CD40127T exhibited 16S rRNA gene sequence similarity values below 96.29% with the rest of Nocardioides species. The G+C content of the genomic DNA was 69.7 mol%. The predominant fatty acid was iso-C16:0 and the major menaquinone was MK-8 (H4) according to the genus Nocardioides. A polyphasic approach using physiological tests, fatty acid profiles, DNA base ratios and DNA-DNA hybridization showed that isolate CD40127T belongs to a novel species within the genus Nocardioides, for which the name Nocardioides albertani is proposed. The type strain is CD40127T (=DSM 25218T =CECT 8014T). |
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