Optical thermal cycler for use as a fluorimetric plate reader to estimate DNA concentrations
Recent developments in PCR techniques provide the opportunity to quantify low copy numbers of specific nucleic acid sequences by quantitative, real-time PCR (1). The use of quantitative, real-time PCR is spreading due to its accuracy and relative simplicity. As a consequence, the number of laboratories equipped with an optical thermal cycler is increasing.
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Main Authors: | , |
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Format: | artículo biblioteca |
Language: | English |
Published: |
Eaton Publications
2003
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Online Access: | http://hdl.handle.net/10261/58622 http://dx.doi.org/10.13039/501100006280 |
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Summary: | Recent developments in PCR techniques provide the opportunity to quantify low copy numbers of specific nucleic acid sequences by quantitative, real-time PCR (1). The use of quantitative, real-time PCR is spreading due to its accuracy and relative simplicity. As a consequence, the number of laboratories equipped with an optical thermal cycler is increasing. |
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