Recombinant West Nile virus envelope protein E and domain III expressed in insect larvae protects mice against West Nile disease
In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infectivity in cell culture and in suckling mice. All vaccinated animals were fully protected when challenged with neurovirulent WNV NY99. Passive transfer of protective antibodies from immunized mothers to their offspring occurred both by transplacental and lactation routes. These results indicate that the insect-derived antigens tested may constitute potential vaccine candidates to be further evaluated. © 2010 Elsevier Ltd.
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
2011
|
| Online Access: | http://hdl.handle.net/20.500.12792/6037 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | In this study, West Nile virus (WNV) envelope (rE) protein and its domain III (rDIII) were efficiently expressed in a cost-effective system based on insect larvae as non-fermentative living biofactories. Mice immunized with the partially purified rE or rDIII elicited high antibodies titers that neutralized viral infectivity in cell culture and in suckling mice. All vaccinated animals were fully protected when challenged with neurovirulent WNV NY99. Passive transfer of protective antibodies from immunized mothers to their offspring occurred both by transplacental and lactation routes. These results indicate that the insect-derived antigens tested may constitute potential vaccine candidates to be further evaluated. © 2010 Elsevier Ltd. |
|---|