Use of enzymatic cDNA amplification as a method of detection of bean yellow mosaic virus
The C-terminal region of bean yellow mosaic virus (BYMV) coat protein gene was selected for the design of oligonucleotide primers. Reverse transcription of viral RNA present in sap of virus-infected plants followed by polymerase chain reaction (RT-PCR) was performed for amplification of a 449 baise pairs fragment. These primers supported BYMV amplification, but did not support amplification on both bean common mosaic virus (BCMV) and potato virus Y (PVY) templates. © 1993 Koninklijke Nederlandse Planteziektenkundige Vereniging.
Main Authors: | , , , , |
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Format: | journal article biblioteca |
Language: | eng |
Published: |
1993
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Online Access: | http://hdl.handle.net/20.500.12792/5830 |
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Summary: | The C-terminal region of bean yellow mosaic virus (BYMV) coat protein gene was selected for the design of oligonucleotide primers. Reverse transcription of viral RNA present in sap of virus-infected plants followed by polymerase chain reaction (RT-PCR) was performed for amplification of a 449 baise pairs fragment. These primers supported BYMV amplification, but did not support amplification on both bean common mosaic virus (BCMV) and potato virus Y (PVY) templates. © 1993 Koninklijke Nederlandse Planteziektenkundige Vereniging. |
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