Response of IFN-γ and IL-4 in mice inoculated with rabies virus recombinant G protein
The aim of this study was to compare the performance of IFN-γ and IL-4 in mice inoculated with recombinant rabies virus G protein that was expressed either in transgenic maize or baculovirus. For this purpose, groups of mice were inoculated as follows Group 1, inactivated rabies vaccine im; Group 2, plant-derived recombinant G protein orally; Group 3, baculovirus-expressed G protein im; Group 4, baculovirus-expressed G protein orally; and group 5, untransformed maize orally. The levels of IFN-γ and IL-4 and specific antibodies were evaluated every 15 d. A challenge was performed at d 60 post-inoculation (pi). Groups 1 and 3 promoted the best humoral response. On the other hand, the results showed that the same groups showed the best levels of IFN-γ at d 10 pi; while the pick of IL4 was detected at d 15 pi. For the survival study, 83 % of the mice immunized with inactivated vaccine, maize or im baculovirus extracts survived viral infection. The recombinant rabies G protein expressed in baculovirus promoted IFN-γ and antibodies in a similar way to the inactivated rabies vaccine. In spite of this, mice fed with transgenic maize survived to the challenge at the same percentage than group 3.
| Main Authors: | , , , , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
2015
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| Online Access: | http://hdl.handle.net/20.500.12792/3873 |
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| Summary: | The aim of this study was to compare the performance of IFN-γ and IL-4 in mice inoculated with recombinant rabies virus G protein that was expressed either in transgenic maize or baculovirus. For this purpose, groups of mice were inoculated as follows Group 1, inactivated rabies vaccine im; Group 2, plant-derived recombinant G protein orally; Group 3, baculovirus-expressed G protein im; Group 4, baculovirus-expressed G protein orally; and group 5, untransformed maize orally. The levels of IFN-γ and IL-4 and specific antibodies were evaluated every 15 d. A challenge was performed at d 60 post-inoculation (pi). Groups 1 and 3 promoted the best humoral response. On the other hand, the results showed that the same groups showed the best levels of IFN-γ at d 10 pi; while the pick of IL4 was detected at d 15 pi. For the survival study, 83 % of the mice immunized with inactivated vaccine, maize or im baculovirus extracts survived viral infection. The recombinant rabies G protein expressed in baculovirus promoted IFN-γ and antibodies in a similar way to the inactivated rabies vaccine. In spite of this, mice fed with transgenic maize survived to the challenge at the same percentage than group 3. |
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