Evaluation of different scarification methods to remove hardseededness in Trifolium subterraneum and Medicago polymorpha accessions of the Spanish base genebank
This work aimed to establish operative methods for removing hardseededness in the viability monitoring tests of the Medicago polymorpha and Trifolium subterraneum accessions preserved in the Spanish base genebank. The following scarification methods were assayed on seeds from one population of each species immersion in sulfuric acid, exposure to liquid nitrogen (LN), seed coat abrasion with sand paper, centrifugal stirring of seeds with sand and seed coat burning. The most efficient scarification treatments for M. polymorpha were immersion in sulfuric acid for 15-30 minutes and seed coat burning. In addition to these treatments, immersion in LN after placement at -18°C and two minutes of centrifugal stirring with sand completely removed hardseededness in the T. subterraneum population without causing seed damage. LN exposure produced cotyledon breakage in M. polymorpha and the stirring treatment had no effect on this species. A number of T. subterraneum accessions stored in the CRF genebank were pre-treated by centrifugal stirring with sand for two minutes and the remaining hard seeds were scarified by seed coat burning. This combination obtained good results in the evaluation of seed viability but the effectiveness of the pre-treatment varied greatly depending on the population assayed.
| Main Authors: | , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
2004
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| Online Access: | http://hdl.handle.net/20.500.12792/2647 |
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| Summary: | This work aimed to establish operative methods for removing hardseededness in the viability monitoring tests of the Medicago polymorpha and Trifolium subterraneum accessions preserved in the Spanish base genebank. The following scarification methods were assayed on seeds from one population of each species immersion in sulfuric acid, exposure to liquid nitrogen (LN), seed coat abrasion with sand paper, centrifugal stirring of seeds with sand and seed coat burning. The most efficient scarification treatments for M. polymorpha were immersion in sulfuric acid for 15-30 minutes and seed coat burning. In addition to these treatments, immersion in LN after placement at -18°C and two minutes of centrifugal stirring with sand completely removed hardseededness in the T. subterraneum population without causing seed damage. LN exposure produced cotyledon breakage in M. polymorpha and the stirring treatment had no effect on this species. A number of T. subterraneum accessions stored in the CRF genebank were pre-treated by centrifugal stirring with sand for two minutes and the remaining hard seeds were scarified by seed coat burning. This combination obtained good results in the evaluation of seed viability but the effectiveness of the pre-treatment varied greatly depending on the population assayed. |
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