In vitro culture selection increases glyphosate tolerance in barley
In vitro culture of barley calluses has been used to produce plants with increased glyphosate tolerance. Calluses from immature embryos of barley Hordeum vulgare L. 'Jeff') were cultured on Murashige and Skoog medium with 10-6, 10-5, 10-4, 5x10-4, 10-3 or 10-2 M glyphosate for one, four or thirty months. Plants were regenerated from calluses maintained in glyphosate medium at 10-6, 10-5 or 10-4 M for four months, at 10-5 or 5x10-4 M for one month and at 10-5 M for thirty months. The progeny of each regenerated plant was analyzed for response to glyphosate. Some progenies showed increased tolerance to glyphosate.
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| Main Authors: | , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
1996
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| Online Access: | http://hdl.handle.net/20.500.12792/1210 |
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| Summary: | In vitro culture of barley calluses has been used to produce plants with increased glyphosate tolerance. Calluses from immature embryos of barley Hordeum vulgare L. 'Jeff') were cultured on Murashige and Skoog medium with 10-6, 10-5, 10-4, 5x10-4, 10-3 or 10-2 M glyphosate for one, four or thirty months. Plants were regenerated from calluses maintained in glyphosate medium at 10-6, 10-5 or 10-4 M for four months, at 10-5 or 5x10-4 M for one month and at 10-5 M for thirty months. The progeny of each regenerated plant was analyzed for response to glyphosate. Some progenies showed increased tolerance to glyphosate. |
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