QTL analysis
The study of the intraspecific natural variation existing in Arabidopsis provides a useful resource for the dissection of its genome at the functional, ecological, and evolutionary levels. A major step in these studies is the identification and location of quantitative trait loci (QTLs) determining the variation for trait(s) of interest, so-called QTL analysis. Here we provide general methods to achieve this goal, including (1) the selection of wild accessions that can serve as parental lines for QTL mapping; (2) the development of mapping populations; (3) the construction of the required genome-wide linkage maps of the populations; and (4) the mapping and characterization of QTLs affecting the trait(s).
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Springer Nature
2006
|
| Subjects: | Arabidopsis, Genetics, Quantitative trait locus (QTL), Natural variation, Mapping, Recombinant inbred lines (RILs), Marker, Polymorphism, Epistasis, Genetic interaction, Pleiotropy, Linkage, |
| Online Access: | http://hdl.handle.net/20.500.12792/6015 http://hdl.handle.net/10261/295222 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The study of the intraspecific natural variation existing in Arabidopsis provides a useful resource for the dissection of its genome at the functional, ecological, and evolutionary levels. A major step in these studies is the identification and location of quantitative trait loci (QTLs) determining the variation for trait(s) of interest, so-called QTL analysis. Here we provide general methods to achieve this goal, including (1) the selection of wild accessions that can serve as parental lines for QTL mapping; (2) the development of mapping populations; (3) the construction of the required genome-wide linkage maps of the populations; and (4) the mapping and characterization of QTLs affecting the trait(s). |
|---|