Cloning of myelomas and hybridomas in fibrin clots
Myelomas and hybridomas were observed to proliferate normally when trapped in a fibrin clot. The fibrin clot was obtained by including fibrinogen in the cell culture medium and thrombin in the plastic dish. A clot formed within seconds and cloning efficiency was around 100%. This technique has all the advantages of semi-solid medium cloning but avoids toxicity to the cells and the exposure to high temperature associated with the soft-agar cloning technique. © 1988.
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| Main Authors: | , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
1988
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| Subjects: | Cloning, Semi-solid medium, Fibrin clot, Myeloma, Hybridoma, |
| Online Access: | http://hdl.handle.net/20.500.12792/5443 http://hdl.handle.net/10261/295010 |
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| Summary: | Myelomas and hybridomas were observed to proliferate normally when trapped in a fibrin clot. The fibrin clot was obtained by including fibrinogen in the cell culture medium and thrombin in the plastic dish. A clot formed within seconds and cloning efficiency was around 100%. This technique has all the advantages of semi-solid medium cloning but avoids toxicity to the cells and the exposure to high temperature associated with the soft-agar cloning technique. © 1988. |
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