RAPD markers for the identification of Populus species

RAPD markers for the identification of Populus species

Show other versions (1)

Twenty five poplar clones, namely, 5 of Populus nigra, 5 of P. deltoides, 5 of P. alba, 5 of P. tremula, 1 of P. trichocarpa, 3 of P. x canescens and 1 of clone 'Platero' (P. tremula x P. alba 'Bolleana') were screened for random amplified polymorphic DNA (RAPD) markers in order to evaluate the use of RAPD analysis for distinguishing the cited species. One of the markers revealed different banding patterns between species and similar banding patterns for clones of the same species. For hybrids such as P. x canescens and 'Platero', the banding pattern was the same as either P. alba or P. tremula. On the other hand, for other hybrids analysed, such as P. x euroamericana I-214 and P. deltoides x P. alba 7/32 B, the banding pattern differed from both parents. The mentioned marker showed characteristic bands for every species, and in the particular case of P. alba three different groups could be distinguished.

Saved in:
Bibliographic Details
Main Authors: Sánchez, N., Grau, J. M., Manzanera, J. A., Bueno, M. A.
Format: journal article biblioteca
Language:English
Published: J.D. Sauerländer's Verlag 1998
Subjects:Populus, Random amplified polymorphic DNA (RAPD), Fingerprinting, Species identification,
Online Access:http://hdl.handle.net/20.500.12792/4041
http://hdl.handle.net/10261/294541
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Twenty five poplar clones, namely, 5 of Populus nigra, 5 of P. deltoides, 5 of P. alba, 5 of P. tremula, 1 of P. trichocarpa, 3 of P. x canescens and 1 of clone 'Platero' (P. tremula x P. alba 'Bolleana') were screened for random amplified polymorphic DNA (RAPD) markers in order to evaluate the use of RAPD analysis for distinguishing the cited species. One of the markers revealed different banding patterns between species and similar banding patterns for clones of the same species. For hybrids such as P. x canescens and 'Platero', the banding pattern was the same as either P. alba or P. tremula. On the other hand, for other hybrids analysed, such as P. x euroamericana I-214 and P. deltoides x P. alba 7/32 B, the banding pattern differed from both parents. The mentioned marker showed characteristic bands for every species, and in the particular case of P. alba three different groups could be distinguished.

Similar Items