Trout immunoglobulin populations differing in light chains revealed by monoclonal antibodies
Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by M Ab 2H9 represented {reversed tilde equals}20% of the total Ig and contained L chains {reversed tilde equals}26kDa, whereas the trout Ig population defined by MAb 2A1 comprised 11% of the total Ig and possessed L chains {reversed tilde equals}24 kDa. The determinants recognized by these MAbs reside on L chains as determined by ELISA using mildly reduced H and L chains. The size heterogeneity of the L chains was not due to differences in glycosylation. Partial peptide maps showed structural differences between both L chains. Both Ig populations were found in each of the 30 trout sera tested. These data extend the existence of L chains diversity in fish, recently described in catfish, to other teleosts. © 1991.
| Main Authors: | , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
1991
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| Online Access: | http://hdl.handle.net/20.500.12792/4017 http://hdl.handle.net/10261/294522 |
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| Summary: | Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by M Ab 2H9 represented {reversed tilde equals}20% of the total Ig and contained L chains {reversed tilde equals}26kDa, whereas the trout Ig population defined by MAb 2A1 comprised 11% of the total Ig and possessed L chains {reversed tilde equals}24 kDa. The determinants recognized by these MAbs reside on L chains as determined by ELISA using mildly reduced H and L chains. The size heterogeneity of the L chains was not due to differences in glycosylation. Partial peptide maps showed structural differences between both L chains. Both Ig populations were found in each of the 30 trout sera tested. These data extend the existence of L chains diversity in fish, recently described in catfish, to other teleosts. © 1991. |
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