Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains
The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles obtained for each of the techniques allowed determination of the degree of polymorphism, genetic diversity and relationships among the strains. Consequently, the discriminatory power and usefulness of the molecular techniques assayed was established. SSR analysis was the most effective method due to its high level of polymorphism, the ease of interpretation of the genetic profiles obtained, and the speed of use. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
| Main Authors: | , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Oxford University Press
2001
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| Subjects: | Random amplified polymorphic DNA, Simple sequence repeat, Cleaved amplified polymorphic sequence, Genetic variability, Molecular typing, Saccharomyces cerevisiae, |
| Online Access: | http://hdl.handle.net/20.500.12792/3401 http://hdl.handle.net/10261/294325 |
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| Summary: | The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles obtained for each of the techniques allowed determination of the degree of polymorphism, genetic diversity and relationships among the strains. Consequently, the discriminatory power and usefulness of the molecular techniques assayed was established. SSR analysis was the most effective method due to its high level of polymorphism, the ease of interpretation of the genetic profiles obtained, and the speed of use. © 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. |
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