Lens culinaris, Phaseolus vulgaris and Vicia faba lectins specifically trigger IL-8 production by the human colon carcinoma cell line Caco-2
Cultured Caco-2 cells were stimulated with Lens culinaris, Phaseolus vulgaris and Vicia faba lectins. The production of IL-1, IL-6, IL-8 and MCP-1 was measured by ELISA and RT-PCR. IL-8 production appeared to be specifically triggered upon stimulation with all three lectins used, since none of the other cytokines tested were produced. The IL-8 secreted may induce the extravasation of activated neutrophils and generate tissue damage. A similar mechanism may be implicated in the lesions observed after infection by some enteric pathogens, with lectin-like domains on their membrane. Finally, this model may be suitable one to study the regulation of IL-8 production. (C) 2000 Academic Press.
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| Main Authors: | , , , , , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
2000
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| Subjects: | Caco-2, IL-8, Lectins, |
| Online Access: | http://hdl.handle.net/20.500.12792/3836 http://hdl.handle.net/10261/294164 |
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| Summary: | Cultured Caco-2 cells were stimulated with Lens culinaris, Phaseolus vulgaris and Vicia faba lectins. The production of IL-1, IL-6, IL-8 and MCP-1 was measured by ELISA and RT-PCR. IL-8 production appeared to be specifically triggered upon stimulation with all three lectins used, since none of the other cytokines tested were produced. The IL-8 secreted may induce the extravasation of activated neutrophils and generate tissue damage. A similar mechanism may be implicated in the lesions observed after infection by some enteric pathogens, with lectin-like domains on their membrane. Finally, this model may be suitable one to study the regulation of IL-8 production. (C) 2000 Academic Press. |
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