Molecular characterization and expression of porcine Siglec-5

In this study we describe the characterization of the porcine orthologue of Siglec-5. A cDNa clone was obtained from a porcine cDNa library derived from swine small intestine which encodes a 555 a-a type 1 transmembrane protein with sequence homology to human Siglec-5. This protein consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail with two tyrosine-based signalling motifs. When expressed as a recombinant protein fused to the Fc region of human IgG1, porcine Siglec-5 was able to bind porcine red blood cells in a sialic acid-dependent manner. Monoclonal antibodies (mAb) were developed against porcine Siglec-5 and used to analyse its expression in bone marrow and blood cells, and lymphoid tissues. Porcine Siglec-5 expression was mainly restricted to myelomonocytic cells and their precursors, being detected also, although at low levels, on plasmacytoid dendritic cells and B lymphocytes. In lymphoid tissues, ellipsoids of the spleen and subcapsular and medullar sinuses of lymph nodes were positive for Siglec-5. These mAbs were able to precipitate, from granulocyte lysates, a protein of approximately 85. kDa under non-reducing conditions, indicating that porcine Siglec-5 is expressed as a monomer in the plasma membrane. © 2013 Elsevier Ltd.

Bibliographic Details

Main Authors:	Escalona, Z., Álvarez, B., Uenishi, H., Toki, D., Yuste, M., Revilla Calvo, Concepción, Gómez del Moral, M., Alonso, F., Ezquerra Martínez, Ángel, Domínguez, J.
Format:	journal article biblioteca
Language:	English
Published:	Elsevier 2014
Subjects:	Siglec-5, Monoclonal antibody, Swine, Myeloid cells, Plasmacytoid DCs,
Online Access:	http://hdl.handle.net/20.500.12792/1207 http://hdl.handle.net/10261/293322
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