Defective interfering RNAs and defective viruses associated with multipartite RNA viruses of plants
Defective interfering (DI) RNAs and defective viruses have been described for a variety of multipartite RNA viruses of plants. At present, the DI RNAs of broad bean, mottle bromovirus (BBMV) have been the most characterized defective elements of multipartite viruses. Several naturally occurring and artificial DI RNAs derived from BBMV RNA2 have been studied to determine the minimum requirements in the RNA sequence and the presence of an open reading frame (ORF) for efficient accumulation of the defective molecule. Sequence/structural elements of BBMV RNA that may be involved in DI RNA formation have been analysed as well. Additional DI RNAs found associated with members of the Bromoviridae family and other virus groups as well as defective viruses are also described. Several general features of DI RNAs and defective viruses derived from multipartite RNA viruses of plants are becoming apparent. In general, for a given virus, the defective element appears to be derived preferentially from only one of the RNA components. DI RNAs are formed via simple, single deletions that preserve some form of an ORF. Finally, when selective conditions have been altered multipartite RNA viruses can form defective viruses quite rapidly by deleting substantial portions of the viral genome. This last point indicates that the entire viral genome is subject to continual selection pressure.
| Main Authors: | , , |
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| Format: | artículo de revisión biblioteca |
| Language: | English |
| Published: |
Elsevier
1996
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| Subjects: | Defective viruses, DI RNAs, Multipartite plant, RNA viruse, |
| Online Access: | http://hdl.handle.net/20.500.12792/1981 http://hdl.handle.net/10261/292386 |
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| Summary: | Defective interfering (DI) RNAs and defective viruses have been described for a variety of multipartite RNA viruses of plants. At present, the DI RNAs of broad bean, mottle bromovirus (BBMV) have been the most characterized defective elements of multipartite viruses. Several naturally occurring and artificial DI RNAs derived from BBMV RNA2 have been studied to determine the minimum requirements in the RNA sequence and the presence of an open reading frame (ORF) for efficient accumulation of the defective molecule. Sequence/structural elements of BBMV RNA that may be involved in DI RNA formation have been analysed as well. Additional DI RNAs found associated with members of the Bromoviridae family and other virus groups as well as defective viruses are also described. Several general features of DI RNAs and defective viruses derived from multipartite RNA viruses of plants are becoming apparent. In general, for a given virus, the defective element appears to be derived preferentially from only one of the RNA components. DI RNAs are formed via simple, single deletions that preserve some form of an ORF. Finally, when selective conditions have been altered multipartite RNA viruses can form defective viruses quite rapidly by deleting substantial portions of the viral genome. This last point indicates that the entire viral genome is subject to continual selection pressure. |
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