Effect of the addition of Micrococcus sp. INIA 528 milk cultures and curds on cheese proteolysis and lipolysis

Semi-hard cheeses were manufactured at laboratory scale from milk inoculated at 2% with a fresh culture of Micrococcus sp. INIA 528, or by mixing high enzymatic activity (HEA) curds, made with this strain, at 10 or 20% with standard curds. Micrococci counts increased slightly during the first hours of manufacture and decreased gradually thereafter. Increases in proteolysis values of 63% in cheese from milk inoculated with micrococci, and of 72% in cheese made with 20% HEA curds, with respect to control cheese were recorded after ripening for 14 days. Tributyrin esterase was detected from day 1 in cheese made from milk inoculated with micrococci and in cheeses with added HEA curds. However, only the addition of HEA curds enhanced lipolysis significantly, with a maximum increase of 85% for cheese made with 20% HEA curds after 14 days. Milk inoculation with micrococci and addition of HEA curds showed to be useful tools for the acceleration of cheese ripening.

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Bibliographic Details
Main Authors: Morales, P., Gaya Sicilia, María Pilar, Núñez Gutiérrez, Manuel
Format: artículo biblioteca
Language:English
Published: AVA Agrar-Verlag Allgäu 2005
Online Access:http://hdl.handle.net/20.500.12792/2984
http://hdl.handle.net/10261/290314
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Summary:Semi-hard cheeses were manufactured at laboratory scale from milk inoculated at 2% with a fresh culture of Micrococcus sp. INIA 528, or by mixing high enzymatic activity (HEA) curds, made with this strain, at 10 or 20% with standard curds. Micrococci counts increased slightly during the first hours of manufacture and decreased gradually thereafter. Increases in proteolysis values of 63% in cheese from milk inoculated with micrococci, and of 72% in cheese made with 20% HEA curds, with respect to control cheese were recorded after ripening for 14 days. Tributyrin esterase was detected from day 1 in cheese made from milk inoculated with micrococci and in cheeses with added HEA curds. However, only the addition of HEA curds enhanced lipolysis significantly, with a maximum increase of 85% for cheese made with 20% HEA curds after 14 days. Milk inoculation with micrococci and addition of HEA curds showed to be useful tools for the acceleration of cheese ripening.