Two-Dimensional Liquid Chromatography in Metabolomics and Lipidomics
Multidimensional separation systems have arisen in the last years to overcome certain limitations of the classical one-dimensional separations. Multidimensional analytical approaches achieve a greater separation power, a crucial aspect when dealing with highly complex samples, as in metabolomics and lipidomics. Online comprehensive two-dimensional chromatography is a particularly interesting mode when pursuing untargeted analysis, which allows for separating, and consequently identifying a more extensive number of analytes. This chapter aims to summarize current applications of 2D-LC to the fields of metabolomics and lipidomics, and setups of different separation mechanism that are being employed, showing the most suitable combinations of chromatographic modes, depending on the target compounds.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | capítulo de libro biblioteca |
| Language: | English |
| Published: |
Springer
2020-11-01
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| Subjects: | Lipidomics, Metabolomics, Two-dimensional liquid chromatography, LC×LC-MS, Comprehensive, |
| Online Access: | http://hdl.handle.net/10261/225094 http://dx.doi.org/10.13039/501100004837 |
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| Summary: | Multidimensional separation systems have arisen in the last years to overcome certain limitations of the classical one-dimensional separations. Multidimensional analytical approaches achieve a greater separation power, a crucial aspect when dealing with highly complex samples, as in metabolomics and lipidomics. Online comprehensive two-dimensional chromatography is a particularly interesting mode when pursuing untargeted analysis, which allows for separating, and consequently identifying a more extensive number of analytes. This chapter aims to summarize current applications of 2D-LC to the fields of metabolomics and lipidomics, and setups of different separation mechanism that are being employed, showing the most suitable combinations of chromatographic modes, depending on the target compounds. |
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