Two-Dimensional Liquid Chromatography in Metabolomics and Lipidomics
Multidimensional separation systems have arisen in the last years to overcome certain limitations of the classical one-dimensional separations. Multidimensional analytical approaches achieve a greater separation power, a crucial aspect when dealing with highly complex samples, as in metabolomics and lipidomics. Online comprehensive two-dimensional chromatography is a particularly interesting mode when pursuing untargeted analysis, which allows for separating, and consequently identifying a more extensive number of analytes. This chapter aims to summarize current applications of 2D-LC to the fields of metabolomics and lipidomics, and setups of different separation mechanism that are being employed, showing the most suitable combinations of chromatographic modes, depending on the target compounds.
Main Authors: | , , |
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Other Authors: | |
Format: | capítulo de libro biblioteca |
Language: | English |
Published: |
Springer
2020-11-01
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Subjects: | Lipidomics, Metabolomics, Two-dimensional liquid chromatography, LC×LC-MS, Comprehensive, |
Online Access: | http://hdl.handle.net/10261/225094 http://dx.doi.org/10.13039/501100004837 |
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Summary: | Multidimensional separation systems have arisen in the last years to overcome certain limitations of the classical one-dimensional separations. Multidimensional analytical approaches achieve a greater separation power, a crucial aspect when dealing with highly complex samples, as in metabolomics and lipidomics. Online comprehensive two-dimensional chromatography is a particularly interesting mode when pursuing untargeted analysis, which allows for separating, and consequently identifying a more extensive number of analytes. This chapter aims to summarize current applications of 2D-LC to the fields of metabolomics and lipidomics, and setups of different separation mechanism that are being employed, showing the most suitable combinations of chromatographic modes, depending on the target compounds. |
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