Stage-specific insecticidal characteristics of a nucleopolyhedrovirus isolate from Chrysodeixis chalcites enhanced by optical brighteners
BACKGROUND: Chrysodeixis chalcites is a major noctuid pest of banana crops in the Canary Islands. The stage-specific susceptibility of this pest to C.¿chalcites single nucleopolyhedrovirus (ChchSNPV-TF1) was determined, as well as the effect of selected optical brighteners as enhancers of primary infection. RESULTS: Susceptibility to ChchSNPV-TF1 occlusion bodies (OBs) decreased as larval stage increased; second instars (L2 ) were 10¿000-fold more susceptible than sixth instars (L6 ). Virus speed of kill was 42¿h faster in L2 than in L6 . OB production increased in late instars; L6 larvae produced 23-fold more OBs than L4 . Addition of 10¿mg¿mL-1 Tinopal enhanced OB pathogenicity by 4.43- to 397-fold depending on instar, whereas 10¿µL¿mL-1 Leucophor resulted in potentiation of OB pathogenicity from 1.46- to 143-fold. Mean time to death decreased by 14 to 26¿h when larvae consumed OBs in mixtures with 10¿mg¿mL-1 Tinopal, or 10¿µL¿mL-1 Leucophor, although in these treatments OB yields were reduced by up to 8.5-fold (Tinopal) or up to 3.8-fold (Leucophor). CONCLUSION: These results have clear applications for the use of ChchSNPV-TF1 as a biological insecticide in control programs against C.¿chalcites in the Canary Islands. © 2013 Society of Chemical Industry. © 2013 Society of Chemical Industry. KEYWORDS: Chrysodeixis¿chalcites, insecticidal activity, nucleopolyhedrovirus, optical brighteners, pest management
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
John Wiley & Sons
2014-05
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| Subjects: | Chrysodeixis chalcites, Nucleopolyhedrovirus, Insecticidal activity, Optical brighteners, Pest management, |
| Online Access: | http://hdl.handle.net/10261/125949 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100003329 |
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| Summary: | BACKGROUND:
Chrysodeixis chalcites is a major noctuid pest of banana crops in the Canary Islands. The stage-specific susceptibility of this pest to C.¿chalcites single nucleopolyhedrovirus (ChchSNPV-TF1) was determined, as well as the effect of selected optical brighteners as enhancers of primary infection.
RESULTS:
Susceptibility to ChchSNPV-TF1 occlusion bodies (OBs) decreased as larval stage increased; second instars (L2 ) were 10¿000-fold more susceptible than sixth instars (L6 ). Virus speed of kill was 42¿h faster in L2 than in L6 . OB production increased in late instars; L6 larvae produced 23-fold more OBs than L4 . Addition of 10¿mg¿mL-1 Tinopal enhanced OB pathogenicity by 4.43- to 397-fold depending on instar, whereas 10¿µL¿mL-1 Leucophor resulted in potentiation of OB pathogenicity from 1.46- to 143-fold. Mean time to death decreased by 14 to 26¿h when larvae consumed OBs in mixtures with 10¿mg¿mL-1 Tinopal, or 10¿µL¿mL-1 Leucophor, although in these treatments OB yields were reduced by up to 8.5-fold (Tinopal) or up to 3.8-fold (Leucophor).
CONCLUSION:
These results have clear applications for the use of ChchSNPV-TF1 as a biological insecticide in control programs against C.¿chalcites in the Canary Islands. © 2013 Society of Chemical Industry.
© 2013 Society of Chemical Industry.
KEYWORDS:
Chrysodeixis¿chalcites, insecticidal activity, nucleopolyhedrovirus, optical brighteners, pest management |
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