A functional virgin olive oil enriched with olive and thyme phenolic compounds improves the expression of cholesterol efflux-related genes: a randomized, cross-over, controlled trial
The consumption of antioxidant-rich foods such as virgin olive oil (VOO) promotes high-density lipoprotein (HDL) anti-atherogenic capacities. Intake of functional VOOs (enriched with olive/thyme phenolic compounds (PCs)) also improves HDL functions, but the gene expression changes behind these benefits are not fully understood. Our aim was to determine whether these functional VOOs could enhance the expression of cholesterol efflux-related genes. In a randomized, double-blind, crossover, controlled trial, 22 hypercholesterolemic subjects ingested for three weeks 25 mL/day of: (1) a functional VOO enriched with olive oil PCs (500 mg/kg); (2) a functional VOO enriched with olive oil (250 mg/kg) and thyme PCs (250 mg/kg; FVOOT), and; (3) a natural VOO (olive oil PCs: 80 mg/kg, control intervention). We assessed whether these interventions improved the expression of cholesterol efflux-related genes in peripheral blood mononuclear cells by quantitative reverse-transcription polymerase chain reactions. The FVOOT intervention upregulated the expression of CYP27A1 (p = 0.041 and p = 0.053, versus baseline and the control intervention, respectively), CAV1 (p = 0.070, versus the control intervention), and LXRβ, RXRα, and PPARβ/δ (p = 0.005, p = 0.005, and p = 0.038, respectively, relative to the baseline). The consumption of a functional VOO enriched with olive oil and thyme PCs enhanced the expression of key cholesterol efflux regulators, such as CYP27A1 and nuclear receptor-related genes.
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Multidisciplinary Digital Publishing Institute
2019-07-26
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| Subjects: | Functional virgin olive oil, Olive oil phenolic compounds, Thyme phenolic compounds, Cholesterol efflux, HDL cholesterol, Transcriptomics, |
| Online Access: | http://hdl.handle.net/10261/193358 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003329 |
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| Summary: | The consumption of antioxidant-rich foods such as virgin olive oil (VOO) promotes
high-density lipoprotein (HDL) anti-atherogenic capacities. Intake of functional VOOs (enriched
with olive/thyme phenolic compounds (PCs)) also improves HDL functions, but the gene expression
changes behind these benefits are not fully understood. Our aim was to determine whether these
functional VOOs could enhance the expression of cholesterol efflux-related genes. In a randomized,
double-blind, crossover, controlled trial, 22 hypercholesterolemic subjects ingested for three weeks
25 mL/day of: (1) a functional VOO enriched with olive oil PCs (500 mg/kg); (2) a functional
VOO enriched with olive oil (250 mg/kg) and thyme PCs (250 mg/kg; FVOOT), and; (3) a natural
VOO (olive oil PCs: 80 mg/kg, control intervention). We assessed whether these interventions
improved the expression of cholesterol efflux-related genes in peripheral blood mononuclear cells by
quantitative reverse-transcription polymerase chain reactions. The FVOOT intervention upregulated
the expression of CYP27A1 (p = 0.041 and p = 0.053, versus baseline and the control intervention,
respectively), CAV1 (p = 0.070, versus the control intervention), and LXRβ, RXRα, and PPARβ/δ
(p = 0.005, p = 0.005, and p = 0.038, respectively, relative to the baseline). The consumption of a functional VOO enriched with olive oil and thyme PCs enhanced the expression of key cholesterol
efflux regulators, such as CYP27A1 and nuclear receptor-related genes. |
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