Molecular analysis of rachis browning in table grapes during storage at 0 ºC: beneficial effect of pretreatments with high levels of Co2
Low temperature storage is an effective technology to extend table grape postharvest life. However, the length of storage is limited by their high susceptibility to fungal decay and the sensitivity of rachis to desiccation and browning. Previous work demonstrated that pretreatment with high CO2 levels (20% CO2 plus 20% O2) for 3 days was able to control fungal decay and reduce rachis browning in table grapes. To better understand the beneficial effect of this pretreatment on maintaining table grape rachis quality at 0 ºC, we analyzed the expression of genes codifying enzymes related to the oxidation of phenolic compounds (phenylalanine ammonia-lyase, VcPAL; and polyphenol oxidase, GPO1), the detoxification of reactive oxygen species (catalase, GCAT; and ascorbate peroxidase, VcAPX) in rachis of treated and non-treated bunches. In addition, and due to their role in senescence, the implication of ethylene and abscisic acid (ABA) in the onset of rachis deterioration was investigated by the expression pattern of key regulatory genes such as ACC synthase (ACS1) and oxidase (ACO1), VvNCED1 and 2.
| Main Authors: | , , , , |
|---|---|
| Format: | capítulo de libro biblioteca |
| Language: | English |
| Published: |
Universidad de Lleida
2012
|
| Online Access: | http://hdl.handle.net/10261/95313 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Low temperature storage is an effective technology to extend table grape postharvest
life. However, the length of storage is limited by their high susceptibility to fungal decay and the sensitivity of rachis to desiccation and browning. Previous work
demonstrated that pretreatment with high CO2
levels (20% CO2 plus 20% O2) for 3
days was able to control fungal decay and reduce rachis browning in table grapes. To
better understand the beneficial effect of this pretreatment on maintaining table grape
rachis quality at 0 ºC, we analyzed the expression of genes codifying enzymes related
to the oxidation of phenolic compounds (phenylalanine ammonia-lyase, VcPAL; and
polyphenol oxidase, GPO1), the detoxification of reactive oxygen species (catalase,
GCAT; and ascorbate peroxidase, VcAPX) in rachis of treated and non-treated bunches.
In addition, and due to their role in senescence, the implication of ethylene and abscisic acid (ABA) in the onset of rachis deterioration was investigated by the expression pattern of key regulatory genes such as ACC synthase (ACS1) and oxidase (ACO1), VvNCED1 and 2. |
|---|