In vivo Antimicrobial Activity Assessment of a Cauliflower By-Product Extract Against Salmonella Typhimurium
The main objective of this work was to study the antimicrobial effect of a cauliflower by-product infusion into an affordable in vivo model (Caenorhabditis elegans). The infusion demonstrated some protective effect on non-infected and infected worms with Salmonella Typhimurium as indicated by higher survival percentile values (75, 50, 25, and 5% percentiles) as compared with those from worms unexposed to the infusion. The antimicrobial effect of the infusion was evaluated on Salmonella intestinal colonization of infected worms (24, 48, and 96 h post-infection). At 96 h post-infection, the concentration of Salmonella was reduced around 2 log cycles in infected cauliflower treated group (p < 0.05) as compared with infected non-cauliflower group. Here we show that cauliflower by-products extend survival and have an antimicrobial effect in an in vivo nematode model, C. elegans, as a previous validation step to longer and costlier farm animal studies.
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Frontiers Media
2020-02-07
|
| Subjects: | Agro-industrial waste, By-product, Cauliflowers, Antimicrobial activity, C. elegans, Salmonella typhimurium, |
| Online Access: | http://hdl.handle.net/10261/200383 http://dx.doi.org/10.13039/501100000780 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The main objective of this work was to study the antimicrobial effect of a cauliflower by-product infusion into an affordable in vivo model (Caenorhabditis elegans). The infusion demonstrated some protective effect on non-infected and infected worms with Salmonella Typhimurium as indicated by higher survival percentile values (75, 50, 25, and 5% percentiles) as compared with those from worms unexposed to the infusion. The antimicrobial effect of the infusion was evaluated on Salmonella intestinal colonization of infected worms (24, 48, and 96 h post-infection). At 96 h post-infection, the concentration of Salmonella was reduced around 2 log cycles in infected cauliflower treated group (p < 0.05) as compared with infected non-cauliflower group. Here we show that cauliflower by-products extend survival and have an antimicrobial effect in an in vivo nematode model, C. elegans, as a previous validation step to longer and costlier farm animal studies. |
|---|