Polynucleotide comprising sequences of wheat gliadins and use thereof for silencing by rnai

[EN] The present invention relates to the specific silencing ofthe a (alpha), B(beta), y (gamma) and co (omega)-gliadins of hard wheat for flour by RNA interference (RNAi) through employment of a polynucIeotide which is transcribed into an hpRNA (hairpin RNA). Furthermore the present invention additionally relates to a vector, cell, plant or seed comprising the polynucIeotide, the expression whereof is specifically directed in particular tissues of wheat seeds through gene expressionregulating sequences such as, for example, the promoter of a gene of y-gliadins or the promoter of the gene encoding for a Dhordein.-meta

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Bibliographic Details
Main Authors: Barro Losada, Francisco, Pistón, Fernando, Gil Humanes, Javier, Martín, Antonio
Format: solicitud de patente biblioteca
Language:Spanish / Castilian
Published: 2010-08-12
Subjects:Gliadinas de trigo, PolynucIeotidos,
Online Access:http://hdl.handle.net/10261/32706
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Description
Summary:[EN] The present invention relates to the specific silencing ofthe a (alpha), B(beta), y (gamma) and co (omega)-gliadins of hard wheat for flour by RNA interference (RNAi) through employment of a polynucIeotide which is transcribed into an hpRNA (hairpin RNA). Furthermore the present invention additionally relates to a vector, cell, plant or seed comprising the polynucIeotide, the expression whereof is specifically directed in particular tissues of wheat seeds through gene expressionregulating sequences such as, for example, the promoter of a gene of y-gliadins or the promoter of the gene encoding for a Dhordein.-meta