Comparative morphometrics and ribosomal DNA sequence analysis of Longidorus orientalis Loof, 1983 (Nematoda: Longidoridae) from Spain and Iran
During recent nematode surveys in a muddy soil around undetermined graminaceous plants in El Rocío, Huelva Province, in southern Spain, and from the rhizosphere of date palm associated with graminaceous vegetation from Abadan, in Khuzestan Province, south-west Iran, populations of Longidorus orientalis were identified. Morphological and morphometrical studies on these populations fit the original description and represent the first report from Spain and Europe. Molecular characterisation of L. orientalis from Spain and Iran, using D2-D3 expansion regions of 28S rRNA, 18S rRNA and ITS-rRNA, is provided. Sequences of the D2-D3 expansion regions and partial 18S genes were analysed using maximum likelihood and Bayesian inference to reconstruct phylogenetic relationships within L. orientalis and other Longidorus species. The results revealed a closer phylogenetic relationship with L. goodeyi for the D2-D3 expansion region and with L. vineacola for the partial 18S region.
| Main Authors: | , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Brill Academic Publishers
2010
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| Online Access: | http://hdl.handle.net/10261/27082 |
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| Summary: | During recent nematode surveys in a muddy soil around
undetermined graminaceous plants in El Rocío, Huelva Province, in
southern Spain, and from the rhizosphere of date palm associated
with graminaceous vegetation from Abadan, in Khuzestan
Province, south-west Iran, populations of Longidorus orientalis
were identified. Morphological and morphometrical studies on
these populations fit the original description and represent the first
report from Spain and Europe. Molecular characterisation of L.
orientalis from Spain and Iran, using D2-D3 expansion regions of
28S rRNA, 18S rRNA and ITS-rRNA, is provided. Sequences of the
D2-D3 expansion regions and partial 18S genes were analysed
using maximum likelihood and Bayesian inference to reconstruct
phylogenetic relationships within L. orientalis and other Longidorus
species. The results revealed a closer phylogenetic relationship
with L. goodeyi for the D2-D3 expansion region and with L.
vineacola for the partial 18S region. |
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