Cytologtical analysis of Elaeis guineensis (tenera) chromosomes.
Cytological analysis were performed on chromosomes of the oil palm Elaeis guineensis of the tenera fruit phenotype using metaphase chromosome spreads. Root tips were obtained from three-to four-week old seedlings and pretreated with 2mM 8-Hydroxyquinoline for 5-6 hours at 18C to increase the metaphase index. Using a protoplast technique, chromosome spreads free of cell wall and cytoplasmic debris were obtained. Cytological analysis on six methaphase spreads showed that oil palm contains 2n32 chromosomes and paired t-tests performed showed significant differences between members. Significant differences may be caused by varying degrees of chromosome condensation between spreads and slide preparations, and also erros incurred during measurement. Based on lengh, chromosome pair No. 1 was assigned to group I, Nos. 2-9 were assigned to group II and Nos. 10-16 to group III Group I consists of the lognest chromosome (10.83 percent of total chromosome length), group II of medium lengh chromkosomes (6.21 percent - 842 of total chromosome length) and group III of medium short chromosomes (3.17 percent - 5.37 percent of total chromosome length).
| Main Authors: | , , |
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| Format: | Texto biblioteca |
| Language: | ng |
| Subjects: | Cariotipos., Citogenetica., Cromosomas., Elaeis guineensis., ténera, Palma de aceite, |
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| Summary: | Cytological analysis were performed on chromosomes of the oil palm Elaeis guineensis of the tenera fruit phenotype using metaphase chromosome spreads. Root tips were obtained from three-to four-week old seedlings and pretreated with 2mM 8-Hydroxyquinoline for 5-6 hours at 18C to increase the metaphase index. Using a protoplast technique, chromosome spreads free of cell wall and cytoplasmic debris were obtained. Cytological analysis on six methaphase spreads showed that oil palm contains 2n32 chromosomes and paired t-tests performed showed significant differences between members. Significant differences may be caused by varying degrees of chromosome condensation between spreads and slide preparations, and also erros incurred during measurement. Based on lengh, chromosome pair No. 1 was assigned to group I, Nos. 2-9 were assigned to group II and Nos. 10-16 to group III Group I consists of the lognest chromosome (10.83 percent of total chromosome length), group II of medium lengh chromkosomes (6.21 percent - 842 of total chromosome length) and group III of medium short chromosomes (3.17 percent - 5.37 percent of total chromosome length). |
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