Isolation of DNA from preserved woods for identification of wood species through genetic analysis regardless of the age of wood production
Species identification of wood provides important information for archaeology, restoration of cultural assets, preventing illegal logging, and more. Wood species are usually identified based on their anatomical features with the use of a microscope. However, this method may not be able to distinguish between anatomically similar species or subspecies. To overcome this problem, wood species need to be identified at the molecular level using DNA sequencing. However, unlike living plant cells, wood is difficult to pulverize using a mortar, and DNA extraction from dried wood is challenging. To solve these problems, we propose a pretreatment method in which wood is pulverized using 60-grit sandpaper and hydrated with water for 2 days. Using this method, we were able to stably amplify the <i>rpoB</i> gene from the extracted DNA of <i>Pinus rigida</i>. In addition, sequence analysis of the <i>rpoB</i> gene revealed six single nucleotide polymorphisms (SNPs), which classified the <i>rpoB</i> sequences in the genus Pinus into five groups. Our data indicate that although these SNPs were not suitable for species identification, they can potentially be used to determine the origin of different wood subspecies or individual samples of wood. Keywords: Monitoring and data collection, Research ID: 3616978
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| Format: | Document biblioteca |
| Language: | English |
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FAO ;
2022
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| Online Access: | https://openknowledge.fao.org/handle/20.500.14283/cc4417en http://www.fao.org/3/cc4417en/cc4417en.pdf |
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| Summary: | Species identification of wood provides important information for archaeology, restoration of cultural assets, preventing illegal logging, and more. Wood species are usually identified based on their anatomical features with the use of a microscope. However, this method may not be able to distinguish between anatomically similar species or subspecies. To overcome this problem, wood species need to be identified at the molecular level using DNA sequencing. However, unlike living plant cells, wood is difficult to pulverize using a mortar, and DNA extraction from dried wood is challenging. To solve these problems, we propose a pretreatment method in which wood is pulverized using 60-grit sandpaper and hydrated with water for 2 days. Using this method, we were able to stably amplify the <i>rpoB</i> gene from the extracted DNA of <i>Pinus rigida</i>. In addition, sequence analysis of the <i>rpoB</i> gene revealed six single nucleotide polymorphisms (SNPs), which classified the <i>rpoB</i> sequences in the genus Pinus into five groups. Our data indicate that although these SNPs were not suitable for species identification, they can potentially be used to determine the origin of different wood subspecies or individual samples of wood.
Keywords: Monitoring and data collection, Research
ID: 3616978 |
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