Mass propagation of a Dendrocalamus asper through seedling macroproliferation for large-scale plantation programs

Dendrocalamus asper is a thick walled edible bamboo species with diversified industrial uses. Vegetative propagation trough culm, rhizome or branch cutting is difficult due to inadequate numbers. Tissue culture requires sophisticated labs, expensive procedure and impractical in many areas of the world. Upon availability, seed germination and seedling macroproliferation could be very simple, inexpensive and advantageous for bamboo propagation which start the physiological age from zero. Current study was therefore carried out over a period of two year from March 2017 to February 2019 to explore the regeneration potentials of D. asper through seed germination and seedling macro-proliferation. Intact or deglumed seeds were soaked in water for 0h, 24h or 48h and sown in pots filled with soil and cow dung under nursery shed (T1) and intact seeds soaked in water mixed with fungicide for 24h were sown in perforated plastic tray filled with soil and cow dung in greenhouse condition (T2) for germination. The fastest germination (7 days) and maximum germination percent (60.3) was in deglumed seeds soaked in water for 24h followed by 40.27% in the same seeds soaked for 48h in T1. Seedlings developed through various pre-sowing treatments were with 4-9 tillers each and macroproliferated six months after germination and grown for another six month for second cycle proliferation. Through macroproliferation, average number of propagules produced were 4 and 8.2 in first cycle and second cycle respectively. Thus 32.8 number of planting materials were produced from a single seedling within one and half year period of time which could be 289.8 through third cycle proliferation at the end of two years. Survival rate of macroproliferated propagules was 86.6% one month after transferring them in the sun. Propagule were ready for third cycle macroproloferation (with 9.06 number of tillers again in each propagule) six months or for planting nine months after the second cycle of macroproliferation. Findings of this study could be a basis for further study and for re-greening the earth for better resilience to climate change adaptations. Keywords: Edible bamboo, deglume seeds, Dendrocalamus asper, seed germination, seedling macroproliferation. ID: 3476549

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Bibliographic Details
Main Author: Chowdhury, S., Islam, H., Azad, A. K., et al.
Format: Article biblioteca
Language:English
Published: FAO ; 2022
Online Access:https://openknowledge.fao.org/handle/20.500.14283/CC1427EN
http://www.fao.org/3/cc1427en/cc1427en.pdf
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Summary:Dendrocalamus asper is a thick walled edible bamboo species with diversified industrial uses. Vegetative propagation trough culm, rhizome or branch cutting is difficult due to inadequate numbers. Tissue culture requires sophisticated labs, expensive procedure and impractical in many areas of the world. Upon availability, seed germination and seedling macroproliferation could be very simple, inexpensive and advantageous for bamboo propagation which start the physiological age from zero. Current study was therefore carried out over a period of two year from March 2017 to February 2019 to explore the regeneration potentials of D. asper through seed germination and seedling macro-proliferation. Intact or deglumed seeds were soaked in water for 0h, 24h or 48h and sown in pots filled with soil and cow dung under nursery shed (T1) and intact seeds soaked in water mixed with fungicide for 24h were sown in perforated plastic tray filled with soil and cow dung in greenhouse condition (T2) for germination. The fastest germination (7 days) and maximum germination percent (60.3) was in deglumed seeds soaked in water for 24h followed by 40.27% in the same seeds soaked for 48h in T1. Seedlings developed through various pre-sowing treatments were with 4-9 tillers each and macroproliferated six months after germination and grown for another six month for second cycle proliferation. Through macroproliferation, average number of propagules produced were 4 and 8.2 in first cycle and second cycle respectively. Thus 32.8 number of planting materials were produced from a single seedling within one and half year period of time which could be 289.8 through third cycle proliferation at the end of two years. Survival rate of macroproliferated propagules was 86.6% one month after transferring them in the sun. Propagule were ready for third cycle macroproloferation (with 9.06 number of tillers again in each propagule) six months or for planting nine months after the second cycle of macroproliferation. Findings of this study could be a basis for further study and for re-greening the earth for better resilience to climate change adaptations. Keywords: Edible bamboo, deglume seeds, Dendrocalamus asper, seed germination, seedling macroproliferation. ID: 3476549