Agrobacterium mediated transformation of Sri Sumrong 60, a Thai cotton variety
Techniques were developed for the induction of embryogenic callus, as well as the transformation and regeneration of Sri Sumrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants on MS based medium containing zeatin and NAA and subculturing on an hormone-free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Sumrong 60 with a synthetic crylA(b) gene was achieved by using Agrobacterium tumefaciens strain, with the addition of 100 mM acetosyringone to the medium during inoculation and co-cultivation steps. PCR amplification was used to verify the presence of ctylA(b) in regenerated plants, thus suggesting the successful transformation of the Thai cotton variety Sri Sumrong 60.
| Main Authors: | , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F30 - Génétique et amélioration des plantes, Gossypium, variété, Agrobacterium tumefaciens, Embryogénèse somatique, cal, transfert de gène, http://aims.fao.org/aos/agrovoc/c_3335, http://aims.fao.org/aos/agrovoc/c_8157, http://aims.fao.org/aos/agrovoc/c_27444, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_24576, http://aims.fao.org/aos/agrovoc/c_24846, |
| Online Access: | http://agritrop.cirad.fr/6577/ |
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| Summary: | Techniques were developed for the induction of embryogenic callus, as well as the transformation and regeneration of Sri Sumrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants on MS based medium containing zeatin and NAA and subculturing on an hormone-free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Sumrong 60 with a synthetic crylA(b) gene was achieved by using Agrobacterium tumefaciens strain, with the addition of 100 mM acetosyringone to the medium during inoculation and co-cultivation steps. PCR amplification was used to verify the presence of ctylA(b) in regenerated plants, thus suggesting the successful transformation of the Thai cotton variety Sri Sumrong 60. |
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