Sugarcane shoot formation in an improved temporary immersion system
A new protocol was established for sugarcane cv. C- 1051-73 shoot formation in a temporary immersion system. The two-step protocol involves shoot formation in 50 ml of culture medium per explant and 1.0 mg 1-1 paclobutrazol for 30 days followed by shoot elongation by exposure to 1.0 mg 1-1 gibberellic acid for 15 days. The multiplication rate was doubled in comparison with the conventional micropropagation protocol (Jiménez et al., 1995) and the cost has been reduced by 46%. Three additional sugarcane varieties have been micropropagated according to this new protocol and results are comparable. Temporary immersion -derived plants have also been compared with conventionally propagated plants in sugarcane fields for more than 9 months and their agricultural indicators of performance are similar. (Author's abstract)
| Main Authors: | , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F02 - Multiplication végétative des plantes, Saccharum, micropropagation, technique de culture, multiplication des plantes, http://aims.fao.org/aos/agrovoc/c_6725, http://aims.fao.org/aos/agrovoc/c_24136, http://aims.fao.org/aos/agrovoc/c_24396, http://aims.fao.org/aos/agrovoc/c_5977, |
| Online Access: | http://agritrop.cirad.fr/6368/ http://agritrop.cirad.fr/6368/1/6368.pdf |
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| Summary: | A new protocol was established for sugarcane cv. C- 1051-73 shoot formation in a temporary immersion system. The two-step protocol involves shoot formation in 50 ml of culture medium per explant and 1.0 mg 1-1 paclobutrazol for 30 days followed by shoot elongation by exposure to 1.0 mg 1-1 gibberellic acid for 15 days. The multiplication rate was doubled in comparison with the conventional micropropagation protocol (Jiménez et al., 1995) and the cost has been reduced by 46%. Three additional sugarcane varieties have been micropropagated according to this new protocol and results are comparable. Temporary immersion -derived plants have also been compared with conventionally propagated plants in sugarcane fields for more than 9 months and their agricultural indicators of performance are similar. (Author's abstract) |
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