Molecular diversity of yam virus Y and identification of banana mild mosaic virus isolates infecting yam (Dioscorea spp.)

Molecular diversity of yam virus Y and identification of banana mild mosaic virus isolates infecting yam (Dioscorea spp.)

Two members of the family Betaflexiviridae associated with yam (Dioscorea spp.) have been described so far: yam latent virus (YLV) and yam virus Y (YVY). However, their geographical distribution and molecular diversity remain poorly documented. Using a nested RT-PCR assay, we detected YVY in D. alata, D. bulbifera, D. cayenensis, D. rotundata, and D. trifida in Guadeloupe, and in D. rotundata in Côte d'Ivoire, thus extending the known host range of this virus and geographical distribution. Using amplicon sequencing, we determined that the molecular diversity of YVY in the yam samples analyzed in this work ranged between 0.0 and 29.1% and that this diversity is partially geographically structured. We also identified three isolates of banana mild mosaic virus (BanMMV) infecting D. alata in Guadeloupe, providing the first evidence for BanMMV infection in yam.

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Bibliographic Details
Main Authors: Diouf, Mame Boucar, Guyader, Sébastien, Nopoly, Marie-Michelle, Gaspard, Olyvia, Filloux, Denis, Candresse, Thierry, Marais, Armelle, Teycheney, Pierre-Yves, Umber, Marie
Format: article biblioteca
Language:eng
Subjects:H20 - Maladies des plantes, identification, virus des végétaux, Dioscorea, Dioscorea cayenensis, Dioscorea rotundata, distribution géographique, séquence nucléotidique, Betaflexiviridae, phylogénie, Dioscorea alata, indexage des virus des plantes, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_2288, http://aims.fao.org/aos/agrovoc/c_2291, http://aims.fao.org/aos/agrovoc/c_2298, http://aims.fao.org/aos/agrovoc/c_5083, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_87c57fca, http://aims.fao.org/aos/agrovoc/c_13325, http://aims.fao.org/aos/agrovoc/c_2289, http://aims.fao.org/aos/agrovoc/c_27034, http://aims.fao.org/aos/agrovoc/c_3406, http://aims.fao.org/aos/agrovoc/c_3081, http://aims.fao.org/aos/agrovoc/c_4027, http://aims.fao.org/aos/agrovoc/c_5159,
Online Access:http://agritrop.cirad.fr/605473/
http://agritrop.cirad.fr/605473/1/s00705-023-05809-3.pdf
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Summary:Two members of the family Betaflexiviridae associated with yam (Dioscorea spp.) have been described so far: yam latent virus (YLV) and yam virus Y (YVY). However, their geographical distribution and molecular diversity remain poorly documented. Using a nested RT-PCR assay, we detected YVY in D. alata, D. bulbifera, D. cayenensis, D. rotundata, and D. trifida in Guadeloupe, and in D. rotundata in Côte d'Ivoire, thus extending the known host range of this virus and geographical distribution. Using amplicon sequencing, we determined that the molecular diversity of YVY in the yam samples analyzed in this work ranged between 0.0 and 29.1% and that this diversity is partially geographically structured. We also identified three isolates of banana mild mosaic virus (BanMMV) infecting D. alata in Guadeloupe, providing the first evidence for BanMMV infection in yam.

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