Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria

Fifty-two slow-growing strains were isolated from root nodules of Calicotome spinosa grown in the Northeast of Algeria and grouped in 24 rep-PCR clusters. One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that all strains were affiliated to Bradyrhizobium. Multi-Locus Sequence Analysis (MLSA) of the atpD, glnII and recA genes and of the 16S-23S rRNA internal transcribed spacer (ITS) showed that these strains formed four divergent clusters: one close to Bradyrhizobium canariense and Bradyrhizobium lupini and three others separate from all the described species, representing three putative new Bradyrhizobium species. A phylogenetic analysis based on the nodC gene sequence affiliated the strains to either of the two symbiovars, genistearum or retamae.

Bibliographic Details

Main Authors:	Salmi, Adouda, Boulila, Farida, Bourebaba, Yasmina, Le Roux, Christine, Belhadi, Djellali, De Lajudie, Philippe
Format:	article biblioteca
Language:	eng
Subjects:	P34 - Biologie du sol, F30 - Génétique et amélioration des plantes, Bradyrhizobium, phylogénie, http://aims.fao.org/aos/agrovoc/c_27138, http://aims.fao.org/aos/agrovoc/c_13325, http://aims.fao.org/aos/agrovoc/c_259,
Online Access:	http://agritrop.cirad.fr/593228/ http://agritrop.cirad.fr/593228/1/Salmi%20%E2%80%A6Le%20Roux%20Christine%202018%20Brady%20Calicotome%20spinosa.pdf
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