Flip-flop method: A new T1-weighted flow-MRI for plants studies
The climate warming implies an increase of stress of plants (drought and torrential rainfall). The understanding of plant behavior, in this context, takes a major importance and sap flow measurement in plants remains a key issue for plant understanding. Magnetic Resonance Imaging (MRI) which is well known to be a powerful tool to access water quantity can be used to measure moving water. We describe a novel flow-MRI method which takes advantage of inflow slice sensitivity. The method involves the slice selectivity in the context of multi slice spin echo sequence. Two sequences such as a given slice is consecutively inflow and outflow sensitive are performed, offering the possiblility to perform slow flow sensitive imaging in a quite straigthforward way. The method potential is demonstrated by imaging both a slow flow measurement on a test bench (as low as 10 μm.s−1) and the Poiseuille's profile of xylemian sap flow velocity in the xylematic tissues of a tomato plant stem.
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | article biblioteca |
| Language: | eng |
| Published: |
PLOS
|
| Subjects: | F60 - Physiologie et biochimie végétale, P40 - Météorologie et climatologie, U30 - Méthodes de recherche, |
| Online Access: | http://agritrop.cirad.fr/587943/ http://agritrop.cirad.fr/587943/1/journal.pone.0194845.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The climate warming implies an increase of stress of plants (drought and torrential rainfall). The understanding of plant behavior, in this context, takes a major importance and sap flow measurement in plants remains a key issue for plant understanding. Magnetic Resonance Imaging (MRI) which is well known to be a powerful tool to access water quantity can be used to measure moving water. We describe a novel flow-MRI method which takes advantage of inflow slice sensitivity. The method involves the slice selectivity in the context of multi slice spin echo sequence. Two sequences such as a given slice is consecutively inflow and outflow sensitive are performed, offering the possiblility to perform slow flow sensitive imaging in a quite straigthforward way. The method potential is demonstrated by imaging both a slow flow measurement on a test bench (as low as 10 μm.s−1) and the Poiseuille's profile of xylemian sap flow velocity in the xylematic tissues of a tomato plant stem. |
|---|