In vitro meristem culture of juvenile and mature Sequoiadendron giganteum
A total of 7000 meristems were used in experiments to investigate the possibility of cloning Sequoiadendron giganteum Buchholz by in vitro meristem culture of juvenile (2-year-old) and mature (100-year-old) ortets. Cultures were initiated on a low-salt medium containing 0.1 mg l−1 1-naphthaleneacetic acid to stimulate meristematic activity. Benzylamino purine (0.01–0.5 mg l−1) inhibited meristematic activity, whereas gibberellic acid (0.01–0.5 mg l−1) had no effect on meristematic development. The mature ortet showed more specific mineral requirements and a lower capacity for cloning than the juvenile ortet. Rooted plants were obtained only from the juvenile clone. There was a marked seasonal effect on meristematic activity, especially for the mature clone, the most active material being obtained during budbreak.
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F60 - Physiologie et biochimie végétale, F02 - Multiplication végétative des plantes, K01 - Foresterie - Considérations générales, |
| Online Access: | http://agritrop.cirad.fr/584111/ http://agritrop.cirad.fr/584111/6/MonteuuisTreePhysiol1987-1.pdf |
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| Summary: | A total of 7000 meristems were used in experiments to investigate the possibility of cloning Sequoiadendron giganteum Buchholz by in vitro meristem culture of juvenile (2-year-old) and mature (100-year-old) ortets. Cultures were initiated on a low-salt medium containing 0.1 mg l−1 1-naphthaleneacetic acid to stimulate meristematic activity. Benzylamino purine (0.01–0.5 mg l−1) inhibited meristematic activity, whereas gibberellic acid (0.01–0.5 mg l−1) had no effect on meristematic development. The mature ortet showed more specific mineral requirements and a lower capacity for cloning than the juvenile ortet. Rooted plants were obtained only from the juvenile clone. There was a marked seasonal effect on meristematic activity, especially for the mature clone, the most active material being obtained during budbreak. |
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