Juvenile Hevea transgenic plants over-expressing transcription factor HbERF-IXc5 develop more latex cells than wild-type material

Mechanical wounding through tapping has long been known to induce laticifer differentiation in Hevea brasiliensis. Recent studies reveal that dehydration, hydrogen peroxide, and jasmonates can similarly play a role in wound-induced laticifer differentiation. The Hevea ethylene response factor gene, HbERF-IXc5, is thought to be an ortholog to ERF1 of Arabidopsis. ERF1 has been suggested as a component of defence responses through the involvement of the ethylene and jasmonic acid signalling pathways. In this study, Agrobacterium tumefaciens-mediated genetic transformation was carried out with this candidate gene. A histological analysis of HbERF-IXc5 lines revealed several changes in anatomy, particularly in terms of the number of laticifer cells. The results suggest that HbERF-IXc5 is a transcription factor driving the expression of some target genes involved in jasmonate-induced laticifer differentiation.

Bibliographic Details

Main Authors:	Lestari, Retno, Rio, Maryannick, Martin, Florence, Leclercq, Julie, Dessailly, Florence, Sanier, Christine, Melliti, Semi, Suharsono, Sony, Montoro, Pascal
Format:	conference_item biblioteca
Language:	eng
Published:	CRRI
Subjects:	F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale,
Online Access:	http://agritrop.cirad.fr/582428/ http://agritrop.cirad.fr/582428/1/Lestari_2016_IRRDB_Conference.pdf
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