Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis
Hevea brasiliensisis cloned by grafting on non-selected rootstock material. In-vitro techniques such as microcutting and somatic embryogenesis were used for propagation of self-rooted plant material. However, these non-commercial techniques are still restricted to a few numbers of clones. To date, long-term somatic embryogenesis is the most promising technique for large-scale propagation. At CIRAD, successful plant regeneration and genetic modification have been obtained for clone PB 260. This paper aims at developing and cryopreserving embryogenic callus lines for clone RRIM 600. Culture conditions were modified for suitable callus growth and embryogenic induction of the low multiplication rate of RRIM600 callus compared with the clone PB 260 one. Several friable callus lines with high embryogenic potential were selected and cryopreserved in large quantity for further field test and functional genomics studies. Application of this technology to other commercial clones can be now considered based on this experience on clones PB 260 and RRIM 600.
| Main Authors: | , , , , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
Prince of Songkla University
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| Subjects: | F02 - Multiplication végétative des plantes, F30 - Génétique et amélioration des plantes, |
| Online Access: | http://agritrop.cirad.fr/579154/ http://agritrop.cirad.fr/579154/1/Establishment%20and%20cryopreservation%20of%20embryogenic%20callus%20lines%20for%20clone%20RRIM%20600%20in%20Hevea%20brasiliensis.pdf |
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| Summary: | Hevea brasiliensisis cloned by grafting on non-selected rootstock material. In-vitro techniques such as microcutting and somatic embryogenesis were used for propagation of self-rooted plant material. However, these non-commercial techniques are still restricted to a few numbers of clones. To date, long-term somatic embryogenesis is the most promising technique for large-scale propagation. At CIRAD, successful plant regeneration and genetic modification have been obtained for clone PB 260. This paper aims at developing and cryopreserving embryogenic callus lines for clone RRIM 600. Culture conditions were modified for suitable callus growth and embryogenic induction of the low multiplication rate of RRIM600 callus compared with the clone PB 260 one. Several friable callus lines with high embryogenic potential were selected and cryopreserved in large quantity for further field test and functional genomics studies. Application of this technology to other commercial clones can be now considered based on this experience on clones PB 260 and RRIM 600. |
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