Isolation of C-glycosyl Xanthones from Coffea pseudozanguebariae and their location
The biochemical composition of leaves from Coffea pseudozanguebariae, a wild caffeine-free coffee species, was determined. Two phenolic compounds were extracted from leaves, separated and characterized. Their structures were elucidated by mass spectrometry, and 1 D and 2D NMR spectroscopy and were shown to be mangiferin (1) and isomangiferin (2), which were the main polyphenol products. Multiphoton fluorescence imaging was performed to visualize polyphenol distribution in leaf cross sections. Consistent biochemical analysis cell imaging techniques on leaves revealed yellow fluorescence in the epidermis and parenchyma cells corresponding to xanthone compounds.
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| Main Authors: | , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F60 - Physiologie et biochimie végétale, U30 - Méthodes de recherche, Q04 - Composition des produits alimentaires, Coffea, composition chimique, composé phénolique, feuille, plante sauvage, Coffea canephora, caféine, fluorescence, spectrométrie de masse, spectroscopie RMN, http://aims.fao.org/aos/agrovoc/c_1720, http://aims.fao.org/aos/agrovoc/c_1794, http://aims.fao.org/aos/agrovoc/c_5772, http://aims.fao.org/aos/agrovoc/c_4243, http://aims.fao.org/aos/agrovoc/c_24126, http://aims.fao.org/aos/agrovoc/c_1723, http://aims.fao.org/aos/agrovoc/c_1183, http://aims.fao.org/aos/agrovoc/c_10935, http://aims.fao.org/aos/agrovoc/c_12421, http://aims.fao.org/aos/agrovoc/c_24405, http://aims.fao.org/aos/agrovoc/c_3081, |
| Online Access: | http://agritrop.cirad.fr/578945/ http://agritrop.cirad.fr/578945/1/Verdeil%20etTalamonetal-2011-ART-Id578945.pdf |
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| Summary: | The biochemical composition of leaves from Coffea pseudozanguebariae, a wild caffeine-free coffee species, was determined. Two phenolic compounds were extracted from leaves, separated and characterized. Their structures were elucidated by mass spectrometry, and 1 D and 2D NMR spectroscopy and were shown to be mangiferin (1) and isomangiferin (2), which were the main polyphenol products. Multiphoton fluorescence imaging was performed to visualize polyphenol distribution in leaf cross sections. Consistent biochemical analysis cell imaging techniques on leaves revealed yellow fluorescence in the epidermis and parenchyma cells corresponding to xanthone compounds. |
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