Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants
This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species.
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| Main Authors: | , , , , , |
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| Format: | book_section biblioteca |
| Language: | eng |
| Published: |
Springer [Etats-Unis]
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| Subjects: | H20 - Maladies des plantes, U30 - Méthodes de recherche, |
| Online Access: | http://agritrop.cirad.fr/576056/ http://agritrop.cirad.fr/576056/1/ID%20576056.pdf |
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| Summary: | This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species. |
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