Development of microsatellite-enriched libraries

Among the molecular markers used for plant genetic studies, microsatellite markers are easy to implement and can provide suitable codominant markers for molecular taxonomy. Here we describe a method to obtain enriched libraries in microsatellite loci from genomic DNA, using capture method with synthetic oligonucleotide probes.

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Bibliographic Details
Main Authors: Vignes, Hélène, Rivallan, Ronan
Format: book_section biblioteca
Language:eng
Published: Humana Press
Subjects:F30 - Génétique et amélioration des plantes, U30 - Méthodes de recherche,
Online Access:http://agritrop.cirad.fr/572397/
http://agritrop.cirad.fr/572397/1/document_572397.pdf
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Summary:Among the molecular markers used for plant genetic studies, microsatellite markers are easy to implement and can provide suitable codominant markers for molecular taxonomy. Here we describe a method to obtain enriched libraries in microsatellite loci from genomic DNA, using capture method with synthetic oligonucleotide probes.