Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

Cold hardening and sucrose treatment improve cryopreservation of date palm meristems

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Date palm (Phoenix dactylifera L.) cv. Khenizi caulogenic meristems were initiated from achlorophyllous leaves excised from in vitro shoot cultures and then proliferated on a specific culture medium supplemented with 70 g dm-3 sucrose. Regeneration rates obtained when using standard vitrification, droplet-vitrification, and encapsulationvitrification protocols reached 26.7, 60.0, and 40.0 %, respectively. Only explants smaller than 3 mm in diameter were found to survive cryogenic treatments. Sucrose preculture, cold hardening and loading solution pretreatments showed significant effects on regeneration rates. Moreover, our results indicate that both sucrose preculture and cold acclimation of explants increased proline content. Cryopreservation of date palm tissue with high proliferation capacity can directly benefit large scale micropropagation projects.

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Bibliographic Details
Main Authors: Fki, Lotfi, Bouaziz, Neila, Chkir, O., Benjemaa-Masmoudi, Raja, Rival, Alain, Swennen, Rony, Drira, Noureddine, Panis, Bart
Format: article biblioteca
Language:eng
Subjects:F02 - Multiplication végétative des plantes, F62 - Physiologie végétale - Croissance et développement, Phoenix dactylifera, culture de méristème, micropropagation, callogénèse, culture in vitro, stockage au froid, congélation, durcissement, régénération in vitro, métabolisme des glucides, vitrification, proline, http://aims.fao.org/aos/agrovoc/c_5791, http://aims.fao.org/aos/agrovoc/c_7043, http://aims.fao.org/aos/agrovoc/c_24136, http://aims.fao.org/aos/agrovoc/c_27475, http://aims.fao.org/aos/agrovoc/c_24135, http://aims.fao.org/aos/agrovoc/c_6484, http://aims.fao.org/aos/agrovoc/c_3091, http://aims.fao.org/aos/agrovoc/c_16185, http://aims.fao.org/aos/agrovoc/c_34114, http://aims.fao.org/aos/agrovoc/c_1299, http://aims.fao.org/aos/agrovoc/c_27625, http://aims.fao.org/aos/agrovoc/c_6223, http://aims.fao.org/aos/agrovoc/c_8007,
Online Access:http://agritrop.cirad.fr/568268/
http://agritrop.cirad.fr/568268/1/document_568268.pdf
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Summary:Date palm (Phoenix dactylifera L.) cv. Khenizi caulogenic meristems were initiated from achlorophyllous leaves excised from in vitro shoot cultures and then proliferated on a specific culture medium supplemented with 70 g dm-3 sucrose. Regeneration rates obtained when using standard vitrification, droplet-vitrification, and encapsulationvitrification protocols reached 26.7, 60.0, and 40.0 %, respectively. Only explants smaller than 3 mm in diameter were found to survive cryogenic treatments. Sucrose preculture, cold hardening and loading solution pretreatments showed significant effects on regeneration rates. Moreover, our results indicate that both sucrose preculture and cold acclimation of explants increased proline content. Cryopreservation of date palm tissue with high proliferation capacity can directly benefit large scale micropropagation projects.

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