Caracterización molecular de poblaciones cubanas de Bemisia tabaci (Gennadius)
An effective control and management of the whitefly Bemisia tabaci (Gennadius) (Hemipterous: Aleyrodidae) requires a previous knowledge about the biotypes circulating in the country. For this work, a molecular study of the population of this insect present in Cuba was carried out. Samples were collected from nine provinces of the country and eight crops of economic interest, including two weed species, and they were evaluated using two microsatellite markers and the sequencing analysis of the mitochondrial cytochrome oxydase I gene (mtCOI). The presence of only one biotype (B) was revealed in all the individuals analyzed. Biotype B possesses a wider host diversity, aggressiveness and capacity of transmission of the disease. Therefore, it is suggested that either biotype B has displaced the native biotype A or that this latter occurs in small populations not found during our surveys. Moreover, the Cuban biotype B showed a range of 95-100% of identity of its mt COI sequence with that of isolates from the Old World or East Hemisphere.
| Main Authors: | , , , , , |
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| Format: | article biblioteca |
| Language: | spa |
| Subjects: | H10 - Ravageurs des plantes, Bemisia tabaci, plante légumière, http://aims.fao.org/aos/agrovoc/c_30755, http://aims.fao.org/aos/agrovoc/c_8165, http://aims.fao.org/aos/agrovoc/c_1997, |
| Online Access: | http://agritrop.cirad.fr/560153/ http://agritrop.cirad.fr/560153/1/document_560153.pdf |
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| Summary: | An effective control and management of the whitefly Bemisia tabaci (Gennadius) (Hemipterous: Aleyrodidae) requires a previous knowledge about the biotypes circulating in the country. For this work, a molecular study of the population of this insect present in Cuba was carried out. Samples were collected from nine provinces of the country and eight crops of economic interest, including two weed species, and they were evaluated using two microsatellite markers and the sequencing analysis of the mitochondrial cytochrome oxydase I gene (mtCOI). The presence of only one biotype (B) was revealed in all the individuals analyzed. Biotype B possesses a wider host diversity, aggressiveness and capacity of transmission of the disease. Therefore, it is suggested that either biotype B has displaced the native biotype A or that this latter occurs in small populations not found during our surveys. Moreover, the Cuban biotype B showed a range of 95-100% of identity of its mt COI sequence with that of isolates from the Old World or East Hemisphere. |
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