Multiplex PCR assay to discriminate four neighbouring species of the Calliptamus genus (Orthoptera: Acrididae) from France

Definition of the genus Calliptamus (Orthoptera: Acrididae) has generated many taxonomic debates. Even now, the existence of different geographical morphs hinders species determination, particularly as concerns females and larvae. Some of these species are observed in southern France and are recognized as potential pests. To circumvent problems of species identification in ecological surveys, we developed a single multiplex PCR method based on mitochondrial Cytochrome Oxydase I diagnostic polymorphisms to differentiate between the four species, Calliptamus italicus, C. wattenwylianus, C. siciliae and C. barbarus, in southern regions of France.

Bibliographic Details

Main Authors:	Blanchet, Elodie, Blondin, Laurence, Gagnaire, P.A., Foucart, Antoine, Vassal, Jean-Michel, Lecoq, Michel
Format:	article biblioteca
Language:	eng
Subjects:	H10 - Ravageurs des plantes, U30 - Méthodes de recherche, L60 - Taxonomie et géographie animales, Calliptamus, identification, espèce, biologie moléculaire, PCR, mitochondrie, technique analytique, taxonomie, http://aims.fao.org/aos/agrovoc/c_1210, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_7280, http://aims.fao.org/aos/agrovoc/c_4891, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_4869, http://aims.fao.org/aos/agrovoc/c_1513, http://aims.fao.org/aos/agrovoc/c_7631, http://aims.fao.org/aos/agrovoc/c_3081,
Online Access:	http://agritrop.cirad.fr/557621/ http://agritrop.cirad.fr/557621/1/document_557621.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!