Evaluation of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus in sera of domestic ruminants in France
This paper describes the sensitivity and specificity of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus (RVFV) from sera of French domestic ruminants. Field samples were collected from mainland France for the known-negative sera (cattle = 191, goats = 119, sheep = 192) and from ruminants of a French overseas territory (Mayotte) for the known-positive sera. A cut-off value of 43% was determined for all species, achieving a sensitivity and specificity of 100% and a concordance of 100% with the species-specific threshold recommended by the manufacturer. Our results demonstrate that this ELISA may be a suitable diagnostic tool for disease surveillance programmes and import/export veterinary certification of French cattle, goats and sheep.
| Main Authors: | , , , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | L73 - Maladies des animaux, ruminant, Virus de la fièvre de la vallée du Rift, Test ELISA, fièvre de la Vallée du Rift, http://aims.fao.org/aos/agrovoc/c_6695, http://aims.fao.org/aos/agrovoc/c_16463, http://aims.fao.org/aos/agrovoc/c_15968, http://aims.fao.org/aos/agrovoc/c_b08d44fd, http://aims.fao.org/aos/agrovoc/c_3081, |
| Online Access: | http://agritrop.cirad.fr/549408/ http://agritrop.cirad.fr/549408/1/document_549408.pdf |
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| Summary: | This paper describes the sensitivity and specificity of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus (RVFV) from sera of French domestic ruminants. Field samples were collected from mainland France for the known-negative sera (cattle = 191, goats = 119, sheep = 192) and from ruminants of a French overseas territory (Mayotte) for the known-positive sera. A cut-off value of 43% was determined for all species, achieving a sensitivity and specificity of 100% and a concordance of 100% with the species-specific threshold recommended by the manufacturer. Our results demonstrate that this ELISA may be a suitable diagnostic tool for disease surveillance programmes and import/export veterinary certification of French cattle, goats and sheep. |
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