Effect of growth regulators on microspore embryogenesis in coconut anthers
The effect of growth regulators on induction of androgenesis in coconut was investigated using seven different growth regulators at various concentrations and combinations. Three auxins (1-naphthalene acetic acid-NAA, indoleacetic acid-IAA, picloram) and three cytokinins (2-isopentyl adenine-2-iP, kinetin, zeatin) were tested either alone or in combination with 2,4-dichlorophenoxyacetic acid (2,4-D), using modified Eeuwens Y3 liquid medium as the basal medium. Among the tested auxins, 100 [mu]M NAA in combination with 100 [mu]M 2,4-D enhanced the production of calli/embryos (123) whereas IAA and picloram showed negative and detrimental effects, respectively, for androgenesis induction over 100 lM 2,4- D alone. Kinetin and 2-iP enhanced the production of calli/ embryos when 100 [mu]M 2,4-D was present in the culture medium. Both cytokinins at 10 [mu]M yielded the highest frequencies of embryos (113 and 93, respectively) whereas zeatin (1 or 2.5 [mu]M) had no impact on microspore embryogenesis. When calli/embryos (produced from different treatments in different experiments) were subcultured in somatic embryo induction medium (Y3 medium containing 66 [mu]M 2,4-D), followed by maturation medium (Y3 medium without growth regulators) and germination medium (Y3 medium containing 5 [mu]M-6-benzyladenine- BA and 0.35 [mu]M gibberellic acid-GA3), plantlets were regenerated at low frequencies (in most treatments ranging from 0% to 7%).
| Main Authors: | , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F60 - Physiologie et biochimie végétale, F30 - Génétique et amélioration des plantes, Cocos nucifera, http://aims.fao.org/aos/agrovoc/c_1716, http://aims.fao.org/aos/agrovoc/c_7345, |
| Online Access: | http://agritrop.cirad.fr/548400/ http://agritrop.cirad.fr/548400/1/document_548400.pdf |
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| Summary: | The effect of growth regulators on induction of androgenesis in coconut was investigated using seven different growth regulators at various concentrations and combinations. Three auxins (1-naphthalene acetic acid-NAA, indoleacetic acid-IAA, picloram) and three cytokinins (2-isopentyl adenine-2-iP, kinetin, zeatin) were tested either alone or in combination with 2,4-dichlorophenoxyacetic acid (2,4-D), using modified Eeuwens Y3 liquid medium as the basal medium. Among the tested auxins, 100 [mu]M NAA in combination with 100 [mu]M 2,4-D enhanced the production of calli/embryos (123) whereas IAA and picloram showed negative and detrimental effects, respectively, for androgenesis induction over 100 lM 2,4- D alone. Kinetin and 2-iP enhanced the production of calli/ embryos when 100 [mu]M 2,4-D was present in the culture medium. Both cytokinins at 10 [mu]M yielded the highest frequencies of embryos (113 and 93, respectively) whereas zeatin (1 or 2.5 [mu]M) had no impact on microspore embryogenesis. When calli/embryos (produced from different treatments in different experiments) were subcultured in somatic embryo induction medium (Y3 medium containing 66 [mu]M 2,4-D), followed by maturation medium (Y3 medium without growth regulators) and germination medium (Y3 medium containing 5 [mu]M-6-benzyladenine- BA and 0.35 [mu]M gibberellic acid-GA3), plantlets were regenerated at low frequencies (in most treatments ranging from 0% to 7%). |
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