![A Cervus genotyping kit based on automated fluorescent multiplex PCR for rapid characterisation of genetic diversity in several deer populations : a tool for wildlife management [Communication orale]](/search/themes/bootstrap3/images/libros.png)
A Cervus genotyping kit based on automated fluorescent multiplex PCR for rapid characterisation of genetic diversity in several deer populations : a tool for wildlife management [Communication orale]
Recent techniques in molecular biology, especially the use of microsatellites markers, and advances in statistics are very useful to characterize the genetic structure of populations and to deduce adapted rules for wildlife management and farming. It becomes possible i) to infer demographic changes, to assess the level of gene flow between populations and the impact of human activities on wild populations; ii) to estimate the impact of farming system on genetic diversity of captive populations, to help the farmers on parentage control in their herds and to set up efficient breeding schemes. In the tropical countries, deer production, including farming and hunting, is economically very important but most often empirical. In this context, we set up a genotyping tool for several tropical deer species using microsatellites markers derived from bovine. Twelve polymorphic microsatellites, labelled with three different fluorochromes, are multiplexed in three PCRs, and analyzed using an ABI 377 sequencer. This genotyping tool was used to characterize populations of two economically important tropical deer species, the Vietnamese sika deer and the rusa deer.
| Main Authors: | , , , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
s.n.
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| Subjects: | L10 - Génétique et amélioration des animaux, Cervidae, génotype, variation génétique, marqueur génétique, http://aims.fao.org/aos/agrovoc/c_1480, http://aims.fao.org/aos/agrovoc/c_3225, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_24030, |
| Online Access: | http://agritrop.cirad.fr/538243/ http://agritrop.cirad.fr/538243/1/document_538243.pdf |
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| Summary: | Recent techniques in molecular biology, especially the use of microsatellites markers, and advances in statistics are very useful to characterize the genetic structure of populations and to deduce adapted rules for wildlife management and farming. It becomes possible i) to infer demographic changes, to assess the level of gene flow between populations and the impact of human activities on wild populations; ii) to estimate the impact of farming system on genetic diversity of captive populations, to help the farmers on parentage control in their herds and to set up efficient breeding schemes. In the tropical countries, deer production, including farming and hunting, is economically very important but most often empirical. In this context, we set up a genotyping tool for several tropical deer species using microsatellites markers derived from bovine. Twelve polymorphic microsatellites, labelled with three different fluorochromes, are multiplexed in three PCRs, and analyzed using an ABI 377 sequencer. This genotyping tool was used to characterize populations of two economically important tropical deer species, the Vietnamese sika deer and the rusa deer. |
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