In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds

In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds

The seed consists of several layers of specialized cell-types that divide and differentiate following a highly regulated programme in time and space. A cytological approach was undertaken in order to study the histo-differentiation at mid-embryogenesis in Medicago truncatula as a model legume, and in Pisum sativum using serial sections of embedded immature seed. Little published information is available about seed development in Medicago species. The observations from this study revealed a number of distinctive features of Medicago seed development and differentiation. Trans-fer cells, involved in nutrient transfer to the embryo, were clearly identified in the thin-walled parenchyma of the innermost integument. Histological Schiff-naphthol enabled carbohydrate accumulation to be followed in the different seed compartments, and revealed the storage protein bodies. Non-radioactive mRNA in situ hybridization, was carried out using mRNA probes from two highly expressed genes encoding the major vicilin and legumin A storage protein types. The timing of mRNA expression was related to that of the corresponding proteins already identified.

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Bibliographic Details
Main Authors: Abirached-Darmency, M., Abdel-Gawwad, M.R., Conejero, Geneviève, Verdeil, Jean-Luc, Thompson, R.
Format: article biblioteca
Language:eng
Subjects:F30 - Génétique et amélioration des plantes, F62 - Physiologie végétale - Croissance et développement, Medicago truncatula, Pisum sativum, semence, différenciation cellulaire, développement embryonnaire, hybridation, arn messager, globuline, maturation, marqueur génétique, expression des gènes, protéine de réserve, http://aims.fao.org/aos/agrovoc/c_26570, http://aims.fao.org/aos/agrovoc/c_5933, http://aims.fao.org/aos/agrovoc/c_6927, http://aims.fao.org/aos/agrovoc/c_2265, http://aims.fao.org/aos/agrovoc/c_2545, http://aims.fao.org/aos/agrovoc/c_3706, http://aims.fao.org/aos/agrovoc/c_4765, http://aims.fao.org/aos/agrovoc/c_3279, http://aims.fao.org/aos/agrovoc/c_4655, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_33936,
Online Access:http://agritrop.cirad.fr/533467/
http://agritrop.cirad.fr/533467/1/document_533467.pdf
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Summary:The seed consists of several layers of specialized cell-types that divide and differentiate following a highly regulated programme in time and space. A cytological approach was undertaken in order to study the histo-differentiation at mid-embryogenesis in Medicago truncatula as a model legume, and in Pisum sativum using serial sections of embedded immature seed. Little published information is available about seed development in Medicago species. The observations from this study revealed a number of distinctive features of Medicago seed development and differentiation. Trans-fer cells, involved in nutrient transfer to the embryo, were clearly identified in the thin-walled parenchyma of the innermost integument. Histological Schiff-naphthol enabled carbohydrate accumulation to be followed in the different seed compartments, and revealed the storage protein bodies. Non-radioactive mRNA in situ hybridization, was carried out using mRNA probes from two highly expressed genes encoding the major vicilin and legumin A storage protein types. The timing of mRNA expression was related to that of the corresponding proteins already identified.

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