Microsatellite DNA markers for the stable fly, Stomoxys calcitrans (Diptera: Muscidae)

Eight polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA library from the stable fly, Stomoxys calcitrans. These loci exhibited four to 15 alleles per locus heterozygosity ranging from 0.19 to 0.84 in the three popular studied from La Réunion island (Indian Ocean). They should therefore be valuable for studying genetic diversity and popuIation structure. Cross-species amplification of these eight loci in the closely allied species Stomoxys niger niger was successful for four of these loci, one of which was monomorphic and one of wich strongly departed from Hardy-Weinberg expectations.

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Bibliographic Details
Main Authors: Gilles, Jérémie, Litrico, Isabelle, Sourrouille, Patricia, Duvallet, Gérard
Format: article biblioteca
Language:eng
Subjects:L50 - Physiologie et biochimie animales, microsatellite, Muscidae, variation génétique, Stomoxys calcitrans, marqueur génétique, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_5001, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_30567, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081,
Online Access:http://agritrop.cirad.fr/524305/
http://agritrop.cirad.fr/524305/1/document_524305.pdf
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Summary:Eight polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA library from the stable fly, Stomoxys calcitrans. These loci exhibited four to 15 alleles per locus heterozygosity ranging from 0.19 to 0.84 in the three popular studied from La Réunion island (Indian Ocean). They should therefore be valuable for studying genetic diversity and popuIation structure. Cross-species amplification of these eight loci in the closely allied species Stomoxys niger niger was successful for four of these loci, one of which was monomorphic and one of wich strongly departed from Hardy-Weinberg expectations.